Cytosplore Viewer is an interactive visual analysis system for the exploration of single cell data published in the Allen Cell Types Database and for a number of the single cell data resources of the Brain Initiative Cell Census Network (BICCN).
Currently supported datasets include the October 2019 data releases in the Allen Cell Types Database of the Allen Institute for Brain Science: nuclear sequencing data of several areas of the human cortex, and single cell sequencing data of the mouse cortex and hippocampus. Also supported is a special mode for cross-species comparison of cell types in the motor cortex between human, marmoset and mouse of BICCN (see Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse below).
Cytosplore Viewer allows an interactive exploration of hierarchies of cell types. One can visualize transcriptome-wide gene expression in combination with metadata of individual cells, perform differential analyses and statistics between manual selections of cells, or between pre-defined clusters throughout the cellular hierarchy and more. Cytosplore Viewer is based on Cytosplore and provides many of the analysis tools presented in the previous Cytosplore publications and is available for download.
TU Delft / LUMC
Project Lead Cytosplore
Cytosplore Viewer is being developed by a team consisting of members from the Leiden Computational Biology Center, the Division of Image Processing at the Leiden University Medical Center, and the Computer Graphics and Visualization Group at the TU Delft in a research project, carried out in close collaboration with Trygve Bakken, Jeremy Miller, Rebecca Hodge, Bosiljka Tasic, Zizhen Yao, Lucas T. Graybuck, Hongkui Zeng, Mike Hawrylycz , and Ed Lein from the Allen Institute for Brain Science.
This work was partially supported by an NWO Gravitation grant:BRAINSCAPES: A Roadmap from Neurogenetics to Neurobiology (NWO: 024.004.012) and NWO AES grants 3DOMICS (NWO: 17126), VANPIRE (NWO: 12720) and Genes in Space (NWO: 12721).
Comparative cellular analysis of motor cortex in human, marmoset, and mouse , Nature, 2021.
Abstract: The primary motor cortex (M1) is essential for voluntary fine motor control and is functionally conserved across mammals. Using high-throughput transcriptomic and epigenomic profiling of over 450,000 single nuclei in human, marmoset monkey, and mouse, we demonstrate a broadly conserved cellular makeup of this region, whose similarity mirrors evolutionary distance and is consistent between the transcriptome and epigenome. The core conserved molecular identity of neuronal and non-neuronal types allowed the generation of a cross species consensus cell type classification and inference of conserved cell type properties across species. Despite overall conservation, many species specializations were apparent, including differences in cell type proportions, gene expression, DNA methylation, and chromatin state. Few cell type marker genes were conserved across species, providing a short list of candidate genes and regulatory mechanisms responsible for conserved features of homologous cell types, such as the GABAergic chandelier cells. This consensus transcriptomic classification allowed the Patch-seq identification of layer 5 (L5) corticospinal Betz cells in non human primate and human and characterization of their highly specialized physiology and anatomy. These findings highlight the robust molecular underpinnings of cell type diversity in M1 across mammals and point to the genes and regulatory pathways responsible for the functional identity of cell types and their species specific adaptations. ...
Conserved Cell Types With Divergent Features in Human Versus Mouse Cortex. , Nature, 2019.
Abstract: Elucidating the cellular architecture of the human cerebral cortex is central to understanding our cognitive abilities and susceptibility to disease. Here we used single-nucleus RNA-sequencing analysis to perform a comprehensive study of cell types in the middle temporal gyrus of human cortex. We identified a highly diverse set of excitatory and inhibitory neuron types that are mostly sparse, with excitatory types being less layer-restricted than expected. Comparison to similar mouse cortex single-cell RNA-sequencing datasets revealed a surprisingly well-conserved cellular architecture that enables matching of homologous types and predictions of properties of human cell types. Despite this general conservation, we also found extensive differences between homologous human and mouse cell types, including marked alterations in proportions, laminar distributions, gene expression and morphology. These species-specific features emphasize the importance of directly studying human brain ...
Shared and Distinct Transcriptomic Cell Types Across Neocortical Areas. , Nature, 2018.
Abstract: The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neocortex, here we analysed 23,822 cells from two areas at distant poles of the mouse neocortex: the primary visual cortex and the anterior lateral motor cortex. We define 133 transcriptomic cell types by deep, single-cell RNA sequencing ...
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Disclaimer: Cytosplore Viewer is a research project between TU Delft and Leiden University Medical Center. We do our best to provide support but cannot guarantee it. If you have any suggestions, problems, want to share your success stories or papers published using Cytosplore Viewer, we would love to get feedback! Please do not hesitate to get in touch.
Cytosplore Viewer is free to use.
If you use Cytosplore Viewer within the scope of a scientific article you must cite the original publications:
Bakken et al. Comparative cellular analysis of motor cortex in human, marmoset and mouse, Nature, 2021.
Hodge, Bakken, et al. Conserved Cell Types With Divergent Features in Human Versus Mouse Cortex, Nature, 2019.
B. Tasic et al., Shared and Distinct Transcriptomic Cell Types Across Neocortical Areas, Nature, 563, pp. 72—78, 2018.
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